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This system involved the administration of anabolic steroids on rats, either orally or by injection (depending on the anabolic steroid being assessed)for 20 weeks, resulting in the establishment of the following parameters: skeletal muscle, muscle tissue density, bone density, liver weight, and skeletal muscle strength for the whole body. The effects of these steroid on the skeletal muscle, liver, and weight was confirmed to be independent of one another, as the weight of different organs increased similarly for all three groups of rats. The anabolic steroid injection significantly increased all three parameters (body weight, skeletal muscle mass, and skeletal muscle strength), while the control group did not increase any of the three parameters. This result suggests that the injection of anabolic steroids results in an increased muscle mass, a concomitant increase in skeletal muscle mass, an increase in skeletal muscle strength, and decreased muscle mass and strength of the rats. The study was performed in accordance with the American Council on Science and Health ("ACSH"). Introduction The purpose of human experiments on animals is to establish relationships that may be used to guide clinical practice (1–3). Experiments involving animals in this regard are generally performed in accordance with the Principles of the Principles of the Animal Welfare Act and regulations (4, 5). The purpose of this section is to report the results of an experiment on humans conducted by the Department of Energy (DOE) at Oak Ridge National Laboratory (ORNL) based on the results summarized in the Principles of the Principles of theAnimal Welfare Act and the regulations (6–9) with an emphasis on the conclusions reached. Materials and Methods The experiment was performed between March 27, 1997 through December 7, 2000, and reported in the October 2000 issue of Journal of the American Council on Science and Health (10). In this report, references to the experimental protocol are cited as follows: Experimental Details Animals Male and female Sprague Dawley rats (Charles River Laboratories), weighing approximately 8–15 g, were used in both feeding experiments. Animals Male and female Sprague Dawley rats (Charles River Laboratories), weighing approximately 8–15 g, were used in both feeding experiments. Animals were housed in individual crates (30 × 30 × 50 cm; Mason Dixon) with a 12-h light/dark cycle maintained at 22 ± 2°C. The rats were supplied with a 1-L tank and a 1,000-watt 12-watt light bulb, and were offered an adequate diet consisting of 2% water (water supplemented with 25% maltodextrin, 50% Osmium Related Article:
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